A mysterious vehicle using a giant magnet steals a highly potent mutagen known as PX-41 from a secret laboratory in the Arctic Circle. The Anti-Villain League (AVL), led by Silas Ramsbottom, sends one of its agents, Lucy Wilde to collect Gru, who was a former super-villain. Meanwhile, at Gru's house, it is one of his daughters, Agnes's birthday and he hosts a party for her. Later, Lucy Wilde uses her lipstick taser to get Gru to the AVL headquarters, as two minions witness the kidnapping and try to save Gru only to end up tagging along. When they make it at the AVL Silas Ramsbottom introduces himself (much to Gru's and the minions amusement) and tells him he was taken there to track down the culprit of the person who stole the lab and recover the mutagen. However, Gru refuses, claiming that he is now a legitimate father and jelly businessman. Longing to resume his criminal life, Dr. Nefario, Gru's friend and assistant, leaves Gru for new employment. This convinces a reluctant Gru to investigate the theft and work with undercover AVL agent Lucy Wilde.